7 098 316

7,098,316 Title:

Human proteins

The present invention relates to novel human proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells and recombinant methods for producing the proteins of the invention. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human secreted proteins.

What is claimed is:

1. An isolated antibody or fragment thereof that specifically binds to a protein selected from the group consisting of: (a) a protein whose amino acid sequence consists ofamino acid residues 1 to 103 of SEQ ID NO:2; (b) a protein whose amino acid sequence consists of a portion of SEQ ID NO:2, wherein said portion is at least 30 contiguous amino acid residues in length; and (c) a protein whose amino acid sequenceconsists of a portion of SEQ ID NO:2, wherein said portion is at least 50 contiguous amino acid residues in length.

2. The antibody or fragment thereof of claim 1 that specifically binds protein (a).

3. The antibody or fragment thereof of claim 1 that specifically binds protein (b).

4. The antibody or fragment thereof of claim 1 that specifically binds protein (c).

5. The antibody or fragment thereof of claim 3 that specifically binds protein (c).

6. The antibody or fragment thereof of claim 3, wherein said protein bound by said antibody or fragment thereof is glycosylated.

7. The antibody or fragment thereof of claim 3, wherein said antibody or fragment thereof is a polyclonal antibody.

8. The antibody or fragment thereof of claim 3, wherein said antibody or fragment thereof is a monoclonal antibody.

9. The antibody or fragment thereof of claim 3, wherein said fragment is an Fab fragment.

10. The antibody or fragment thereof of claim 3, wherein said antibody or fragment thereof is labeled.

11. An isolated cell that produces the antibody of claim 3.

12. A hybridoma that produces the antibody of claim 3.

13. A method of detecting HEMFI85 protein shown in SEQ ID NO:2, in a biological sample comprising: (a) contacting the biological sample with the antibody or fragment thereof of claim 3; (b) allowing said antibody or fragment thereof to bindthe HEMFI85 protein; and, (c) identifying the presence of the HEMFI85 protein bound by said antibody.

14. An isolated antibody or fragment thereof that specifically binds HEMFI85 protein produced by a cell culture wherein the cells in said cell culture comprise a polynucleotide encoding amino acids 1 to 103 of SEQ ID NO:2.

15. The antibody or fragment thereof of claim 14, wherein said antibody or fragment thereof is a monoclonal antibody.

16. The antibody or fragment thereof of claim 14, wherein said antibody or fragment thereof is a polyclonal antibody.

17. The antibody or fragment thereof of claim 14, wherein said fragment is an Fab fragment.

18. The antibody or fragment thereof of claim 14, wherein the amino acid sequence of said HEMFI85 protein consists of amino acid residues 1 to 103 of SEQ ID NO:2.